Category
Basic
Description
This project tests the hypothesis that developing antibodies specific to zebrafish CD5 and IgM will enable the reliable identification and characterization of B1 B-cells in zebrafish. B1 B-cells play a critical role in autoimmunity across species, yet their functions remain poorly characterized in zebrafish (Danio rerio). Zebrafish are widely used as a developmental and immunological model organism due to their genetic similarity to humans as well as their transparent embryos. The lack of species-specific antibodies has hindered the study of B1 B-cells in zebrafish.
This project seeks to develop and validate antibodies against zebrafish CD5 and IgM. This will be accomplished through recombinant protein production, purification of CD5 and IgM antigens, and subsequent antibody generation and validation. This approach will enable the identification and characterization of B1 B-cells in zebrafish.
Characterizing B1 B-Cells in Zebrafish (Danio rerio)
Basic
This project tests the hypothesis that developing antibodies specific to zebrafish CD5 and IgM will enable the reliable identification and characterization of B1 B-cells in zebrafish. B1 B-cells play a critical role in autoimmunity across species, yet their functions remain poorly characterized in zebrafish (Danio rerio). Zebrafish are widely used as a developmental and immunological model organism due to their genetic similarity to humans as well as their transparent embryos. The lack of species-specific antibodies has hindered the study of B1 B-cells in zebrafish.
This project seeks to develop and validate antibodies against zebrafish CD5 and IgM. This will be accomplished through recombinant protein production, purification of CD5 and IgM antigens, and subsequent antibody generation and validation. This approach will enable the identification and characterization of B1 B-cells in zebrafish.
