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Theoretical Proposal

Description

Mosquito borne diseases are a global health concern, requiring effective methods of vector control. Pyriproxyfen (PPF) is an insect growth regulator that prevents larvae from becoming adult mosquitoes. The adults exposed to PPF often have difficulties in reproduction. Pyrethroids, however, are an insecticide meant to kill mosquitoes. Oftentimes PPF and pyrethroids are used together and resistance has become an increasing concern. This study would investigate if a sublethal larval exposure to PPF will cause increased resistance to pyrethroids in adult Culcidine mosquitoes. Two separate mosquito groups would be reared with or without larval PPF exposure. The adults from both groups would be tested for pyrethroid resistance using a CDC bottle bioassay. RNA sequencing would also be conducted to observe the changes in gene expression and regulation after exposure to PPF. This research would provide important insight into improving vector control by reducing resistance.

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Apr 20th, 1:30 PM Apr 20th, 2:00 PM

Sub-Lethal Exposure of Culcicidine Mosquitoes to Pyriproxyfen: A Gateway to Pyrethroid Resistance

Theoretical Proposal

Mosquito borne diseases are a global health concern, requiring effective methods of vector control. Pyriproxyfen (PPF) is an insect growth regulator that prevents larvae from becoming adult mosquitoes. The adults exposed to PPF often have difficulties in reproduction. Pyrethroids, however, are an insecticide meant to kill mosquitoes. Oftentimes PPF and pyrethroids are used together and resistance has become an increasing concern. This study would investigate if a sublethal larval exposure to PPF will cause increased resistance to pyrethroids in adult Culcidine mosquitoes. Two separate mosquito groups would be reared with or without larval PPF exposure. The adults from both groups would be tested for pyrethroid resistance using a CDC bottle bioassay. RNA sequencing would also be conducted to observe the changes in gene expression and regulation after exposure to PPF. This research would provide important insight into improving vector control by reducing resistance.

 

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