Category
Poster - Basic
Description
This experiment focuses on attaching a cholesterol group to the fluorophore, 9,10-anthracenedione. Cholesterol is a structural component of cell membranes, and its lipophilic properties allow it to pass through the phospholipid bilayer.1 Anthraquinones are a class of compounds obtained from natural sources such as fungi, plants, animals, and bacteria and represent the second most important class of dyes. Anthraquinone dyes are composed of 9,10-anthraquinones and can be modified at various positions.2 This reaction uses a cholesterol derivative which has been used as a lipid anchor for hydrophobization of other fluorophores.3 Cholesterol-fluorophore conjugates have been used to examine the functions of cholesterol in cell membranes such as cholesterol organization, trafficking, and lipid interactions.4 Fluorescence spectroscopy is a method of analyzing a compound’s fluorescent properties and is used extensively in biotechnology, flow cytometry, medical diagnostics, DNA sequencing, and genetic analysis.5 This experiment will attempt to synthesize a fluorescent dye that will be able to embed its lipid portion in the phospholipid bilayer while the fluorophore is left exposed.6 Successfully attaching the cholesterol group to the fluorophore suggests the potential for fluorescent dye integration in the cell membrane and cell staining applications. Preliminary results will be discussed during the presentation as well as the implications of future research.
Cholesterol-Fluorophore Bioconjugates
Poster - Basic
This experiment focuses on attaching a cholesterol group to the fluorophore, 9,10-anthracenedione. Cholesterol is a structural component of cell membranes, and its lipophilic properties allow it to pass through the phospholipid bilayer.1 Anthraquinones are a class of compounds obtained from natural sources such as fungi, plants, animals, and bacteria and represent the second most important class of dyes. Anthraquinone dyes are composed of 9,10-anthraquinones and can be modified at various positions.2 This reaction uses a cholesterol derivative which has been used as a lipid anchor for hydrophobization of other fluorophores.3 Cholesterol-fluorophore conjugates have been used to examine the functions of cholesterol in cell membranes such as cholesterol organization, trafficking, and lipid interactions.4 Fluorescence spectroscopy is a method of analyzing a compound’s fluorescent properties and is used extensively in biotechnology, flow cytometry, medical diagnostics, DNA sequencing, and genetic analysis.5 This experiment will attempt to synthesize a fluorescent dye that will be able to embed its lipid portion in the phospholipid bilayer while the fluorophore is left exposed.6 Successfully attaching the cholesterol group to the fluorophore suggests the potential for fluorescent dye integration in the cell membrane and cell staining applications. Preliminary results will be discussed during the presentation as well as the implications of future research.
Comments
Undergraduate