Category

Poster - Basic

Description

This experiment focuses on attaching a cholesterol group to the fluorophore, 9,10-anthracenedione. Cholesterol is a structural component of cell membranes, and its lipophilic properties allow it to pass through the phospholipid bilayer.1 Anthraquinones are a class of compounds obtained from natural sources such as fungi, plants, animals, and bacteria and represent the second most important class of dyes. Anthraquinone dyes are composed of 9,10-anthraquinones and can be modified at various positions.2 This reaction uses a cholesterol derivative which has been used as a lipid anchor for hydrophobization of other fluorophores.3 Cholesterol-fluorophore conjugates have been used to examine the functions of cholesterol in cell membranes such as cholesterol organization, trafficking, and lipid interactions.4 Fluorescence spectroscopy is a method of analyzing a compound’s fluorescent properties and is used extensively in biotechnology, flow cytometry, medical diagnostics, DNA sequencing, and genetic analysis.5 This experiment will attempt to synthesize a fluorescent dye that will be able to embed its lipid portion in the phospholipid bilayer while the fluorophore is left exposed.6 Successfully attaching the cholesterol group to the fluorophore suggests the potential for fluorescent dye integration in the cell membrane and cell staining applications. Preliminary results will be discussed during the presentation as well as the implications of future research.

Comments

Undergraduate

Share

COinS
 
Apr 17th, 1:00 PM

Cholesterol-Fluorophore Bioconjugates

Poster - Basic

This experiment focuses on attaching a cholesterol group to the fluorophore, 9,10-anthracenedione. Cholesterol is a structural component of cell membranes, and its lipophilic properties allow it to pass through the phospholipid bilayer.1 Anthraquinones are a class of compounds obtained from natural sources such as fungi, plants, animals, and bacteria and represent the second most important class of dyes. Anthraquinone dyes are composed of 9,10-anthraquinones and can be modified at various positions.2 This reaction uses a cholesterol derivative which has been used as a lipid anchor for hydrophobization of other fluorophores.3 Cholesterol-fluorophore conjugates have been used to examine the functions of cholesterol in cell membranes such as cholesterol organization, trafficking, and lipid interactions.4 Fluorescence spectroscopy is a method of analyzing a compound’s fluorescent properties and is used extensively in biotechnology, flow cytometry, medical diagnostics, DNA sequencing, and genetic analysis.5 This experiment will attempt to synthesize a fluorescent dye that will be able to embed its lipid portion in the phospholipid bilayer while the fluorophore is left exposed.6 Successfully attaching the cholesterol group to the fluorophore suggests the potential for fluorescent dye integration in the cell membrane and cell staining applications. Preliminary results will be discussed during the presentation as well as the implications of future research.

 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.