Category
Poster - Basic
Description
Anatomical and physiological study of the human body extends beyond the life of an individual, giving us a perspective of the post-mortem period with cadaveric studies. Cadavers serve as a valuable resource by providing tangible access into the intricacies of human anatomy, normal physiology, and how various pathologies affect their implicated tissues, structures, and functions. Numerous techniques allow researchers to identify the effects of pathologies within different organ systems. Among these systems, our current interest involves central nervous system pathologies. Due to their complexity, neurodegenerative diseases typically require multiple studies and characterization to be understood and to distinguish them from similar pathologies. . More specifically, we wish to explore the brain and spinal cord as potential indicators of complex disease, harvesting and utilizing them from donated cadavers. Our research seeks to employ frequently applied staining techniques such as hematoxylin and eosin (H&E) and nervous tissue specific staining, Luxol Fast Blue (LFB) and Golgi staining in combination with immunohistochemical qualitative analysis to mark glia activation and proliferation, and neuronal injury. Changes in neuron morphology that occur in the spines, dendritic arbor, and axon, or within its soma are distinctive characteristics of neuronal pathology. Additionally, the presence of infiltrating cells, gliosis and the rearrangement of neuronal circuits are observed in animal models of neurological conditions at different stages. Therefore, we wish to understand how these staining approaches in various regions of the brain and spinal cord can be used to potentially identify new pathological markers of central nervous system disorders. This research suggests the use of implementing differential staining and immunohistochemical techniques in abnormal post-mortem cadaver brain and spinal cord samples for biomedical research and possible clinical applications.
Investigation of New Pathological Markers in Cadaveric Nervous Tissue
Poster - Basic
Anatomical and physiological study of the human body extends beyond the life of an individual, giving us a perspective of the post-mortem period with cadaveric studies. Cadavers serve as a valuable resource by providing tangible access into the intricacies of human anatomy, normal physiology, and how various pathologies affect their implicated tissues, structures, and functions. Numerous techniques allow researchers to identify the effects of pathologies within different organ systems. Among these systems, our current interest involves central nervous system pathologies. Due to their complexity, neurodegenerative diseases typically require multiple studies and characterization to be understood and to distinguish them from similar pathologies. . More specifically, we wish to explore the brain and spinal cord as potential indicators of complex disease, harvesting and utilizing them from donated cadavers. Our research seeks to employ frequently applied staining techniques such as hematoxylin and eosin (H&E) and nervous tissue specific staining, Luxol Fast Blue (LFB) and Golgi staining in combination with immunohistochemical qualitative analysis to mark glia activation and proliferation, and neuronal injury. Changes in neuron morphology that occur in the spines, dendritic arbor, and axon, or within its soma are distinctive characteristics of neuronal pathology. Additionally, the presence of infiltrating cells, gliosis and the rearrangement of neuronal circuits are observed in animal models of neurological conditions at different stages. Therefore, we wish to understand how these staining approaches in various regions of the brain and spinal cord can be used to potentially identify new pathological markers of central nervous system disorders. This research suggests the use of implementing differential staining and immunohistochemical techniques in abnormal post-mortem cadaver brain and spinal cord samples for biomedical research and possible clinical applications.
Comments
Undergraduate