School of Health Sciences
Biology: Biomedical Sciences
folate, zinc finger protein 410, gene methlation, methotrexate
Amino Acids, Peptides, and Proteins | Biochemical Phenomena, Metabolism, and Nutrition | Genetic Processes | Neurosciences | Nucleic Acids, Nucleotides, and Nucleosides
Locke, Mariko, "Determining the Genomic Localization and Binding Partners of Zinc Finger Protein 410" (2022). Senior Honors Theses. 1226.
The results of a folate deficiency study affecting cognition in mice suggested the altered genes may be controlled by a transcription factor known as Zinc Finger Protein 410 (Zfp410). Due to a lack of literature on Zfp410’s interacting proteins and DNA-binding location, our study aims to further elucidate the role Zfp410 plays in affecting cognition. A custom antibody was used to determine the Zfp410 isoforms present in mouse and rat brains. Moreover, the antibody was used to determine the binding partners of Zfp410 in the brain and locate specific genomic regions/sequences with which it associates in vivo. These results may further elucidate the molecular pathways of Zfp410 in relation to cognition and learning.
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