Publication Date
4-2022
School
School of Health Sciences
Major
Biology: Biomedical Sciences
Keywords
folate, zinc finger protein 410, gene methlation, methotrexate
Disciplines
Amino Acids, Peptides, and Proteins | Biochemical Phenomena, Metabolism, and Nutrition | Genetic Processes | Neurosciences | Nucleic Acids, Nucleotides, and Nucleosides
Recommended Citation
Locke, Mariko, "Determining the Genomic Localization and Binding Partners of Zinc Finger Protein 410" (2022). Senior Honors Theses. 1226.
https://digitalcommons.liberty.edu/honors/1226
Abstract
The results of a folate deficiency study affecting cognition in mice suggested the altered genes may be controlled by a transcription factor known as Zinc Finger Protein 410 (Zfp410). Due to a lack of literature on Zfp410’s interacting proteins and DNA-binding location, our study aims to further elucidate the role Zfp410 plays in affecting cognition. A custom antibody was used to determine the Zfp410 isoforms present in mouse and rat brains. Moreover, the antibody was used to determine the binding partners of Zfp410 in the brain and locate specific genomic regions/sequences with which it associates in vivo. These results may further elucidate the molecular pathways of Zfp410 in relation to cognition and learning.
Included in
Amino Acids, Peptides, and Proteins Commons, Biochemical Phenomena, Metabolism, and Nutrition Commons, Genetic Processes Commons, Neurosciences Commons, Nucleic Acids, Nucleotides, and Nucleosides Commons
