Publication Date


Document Type

Conference Presentation


Cell and Developmental Biology | Cell Biology | Other Cell and Developmental Biology


This presentation was given at the American Society of Microbiology Biodefense Annual Meeting on March 20-23, 2005 in Baltimore, MD. The presentation can be referenced using the following citation:

Monique van Hoek, Debra Anderson, Anne Pingitore, Pavel Vasioutovitch,* Megan Schaffner,* Koryn Johnston,* Allison Jablonski. Infection of Mammalian Hepatocytes by Francisella tularensis LVS. In: American Society of Microbiology Biodefense Annual Meeting; March 20-23, 2005; Baltimore, MD.


Francisella tularensis, the causative agent of Tularemia, is a facultative intracellular parasite. The goal of this project is to examine how F. tularensis infects and replicates within mammalian cells, and the impact of such an infection upon the signaling pathways within the host cell. The murine embryonic hepatocyte cell line TIB-73 serves as our model system for infection by F. tularensis live vaccine strain (LVS). The number of bacteria associated with hepatocytes was quantified via adhesion, invasion and intracellular replication assays. We have determined that a large multiplicity of infection (MOI) of F. tularensis LVS is required to infect the hepatocyte cells. The infective capacity of F. tularensis LVS in hepatocytes was found to be ineffective below an MOI of 0.1. Western blotting was used to determine the presence of various kinases in hepatocytes before infection with F. tularensis LVS.