Local Bacteriological Survey: Isolation and Characterization of Bacteria in Two Lynchburg Parks
College of Arts and Sciences
Biochemistry and Molecular Biology
Primary Subject Area
Hassenpflug, Rachel S., "Local Bacteriological Survey: Isolation and Characterization of Bacteria in Two Lynchburg Parks" (2008). Senior Honors Theses. 48.
Water samples were collected from the bodies of water at Peaks View Park and Percival’s Isle Park in Lynchburg, Virginia in two separate trials per collection site. The samples were filtered to isolate bacteria present in the water. The filters were placed on Trypticase Soy Agar (TSA) and Eosin Methylene Blue (EMB) plates and incubated to obtain colony counts of the bacteria. Subsequently, distinct colonies were selected from the filtration plates and streaked for isolation. Isolates were subjected to a variety of analytical methods and thereby identified by genus and species. Fifteen different species were identified from twenty-three different samples. The samples included an alarming number of E. coli colonies, as well as other enterobacteria and human pathogens.