Publication Date

Spring 4-14-2023


School of Health Sciences


Biochemistry and Molecular Biology


Pharmaceuticals, Chromatography, Analytical Methods, High-Performance Liquid Chromatography, Stationary Phase, Chemical Gradients, Stationary Phase Gradients, Pharmacological Research, Separation Science, Drug Analysis, Drug Development


Complex Mixtures | Medicinal and Pharmaceutical Chemistry | Pharmaceutical Preparations | Pharmaceutics and Drug Design


The separation of mixtures into different components is integral to experimentation and analysis in a multitude of fields. Chromatography is one of the most popular, well-developed, and well-studied methods used to examine the makeup of a mixture. Thus, the improvement of chromatographic procedures directly benefits research across many scientific disciplines. The application of a continuous stationary phase gradient to High-Performance Liquid Chromatography (HPLC) methods has been proposed to improve the separation of complex mixtures that are difficult to achieve with existing separation techniques. By incorporating a gradient stationary phase, analysts will create a more selective mode of separation to improve the efficiency and cost-effectiveness of their research. Particularly, this new protocol will enhance the function of the pharmaceutical industry, which relies heavily on chromatographic methods.