School of Health Sciences
Biochemistry and Molecular Biology
Yeast, Glucose, Rgt2, Casein Kinase, Yck, Glucose Sensing Receptor, Western Blot, Yeast Two-Hybrid
Mohler, Emma E., "The Yeast Glucose Sensing Receptor is Stabilized by Interaction with Casein Kinases" (2022). Senior Honors Theses. 1203.
Many metabolic diseases are rooted in the inability to process glucose or regulate its uptake. These processes can be explored using yeast as a model. Rgt2 is a glucose sensing receptor in yeast, and it detects glucose concentration outside the cell. This receptor is present on the plasma membrane in high glucose conditions but absent in low glucose. Western blot and a yeast two-hybrid assay were used to investigate the relationship between Rgt2 and the plasma membrane-tethered yeast casein kinases, Yck1 and Yck2. This research demonstrated that in high glucose, Rgt2 is stabilized on the plasma membrane by interaction with Yck1 and Yck2. In response to glucose, the Ycks likely phosphorylate the Rgt2 CTD between amino acids 665-696.