College of Arts and Sciences
peroxidase, kudzu, enzyme, biochemical research, chemical activities
Briley, Kathryn, "The Discovery and Analysis of Peroxidase Enzyme in Pueraria montana" (2021). Senior Honors Theses. 1071.
Peroxidase enzymes are used for a variety of industrial and biotechnological applications because of their ease of purification, broad range of chemical activities, and low cost of use. Identification of quality peroxidase sources that are convenient for enzymatic isolation and give way to high yields of product is a desirable pursuit in biochemical research. Kudzu is an excellent candidate for this pursuit as it displays high catalytic activity in screening assays and is found in abundance. This project seeks to determine the enzyme stability, optimal pH conditions, and possible novel chemical activities of peroxidase isolated from kudzu leaves. The methods for this project include standard protein purification protocols, analytical techniques for evaluation of chemical activities, and purity of the resulting preparations.