School of Health Sciences
Biochemistry and Molecular Biology
Chemotherapy, aptamer, Lung Cancer, Norcantharidin
Biology | Chemical and Pharmacologic Phenomena | Medical Cell Biology | Medicine and Health Sciences | Molecular Biology
Fehr, Shannon, "DNA Aptamer-Drug Targeting Chemotherapy: Investigation of Cell Cycle Inhibition via S15 Aptamer – Norcantharidin Complex" (2020). Senior Honors Theses. 1015.
Lung Cancer is the leading cause of cancer related death in both men and women in the United States (Bray et al., 2018). Cancer treatments are often non-specific and kill many dividing cells within a patient causing unwanted side effects. Norcantharidin (NCTD) is a synthetic FDA approved treatment for cancers including non-small cell lung cancers (NSCLC). NCTD suppresses cell proliferation by inhibiting cells from exiting the G2 phase of the cell cycle. Aptamers are short single-stranded DNA or RNA molecules with ligand directed self-annealing capabilities allowing selective binding to specific targets. This paper will discuss the hypothesized effects of using a single-stranded DNA aptamer bound to NCTD to reduce proliferation of A549 cells, an adenocarcinoma cell line often used to study NSCLC (Foster et al., 1998 & Giard et al., 1973).