Methods for the Isolation of Sensory and Primary Cilia—An Overview

Author(s)

Kimberly A. P. Mitchell, Liberty UniversityFollow
Gabor Szabo
Angela de S. Otero

Publication Date

2009

Document Type

Article

Disciplines

Biology | Chemistry

Comments

Published in Methods in Cell Biology, R. Sloboda, ed., Vol. 94, pp. 87–101. Academic Press.

Abstract

Detailed proteomic analyses of mammalian olfactory and rod photoreceptor sensory cilia are now available, providing an inventory of resident ciliary proteins and laying the foundation for future studies of developmental and spatio-temporal changes in the composition of sensory cilia. Cilia purification methods that were elaborated and perfected over several decades were essential for these advances. In contrast, the proteome of primary cilia is yet to be established, because purification procedures for this organelle have been developed only recently. In this chapter we review current techniques for the purification of olfactory and photoreceptor cilia, and evaluate methods designed for the selective isolation of primary cilia.

Recommended Citation

Mitchell, Kimberly A. P.; Szabo, Gabor; and Otero, Angela de S., "Methods for the Isolation of Sensory and Primary Cilia—An Overview" (2009). Faculty Publications and Presentations. 105.
https://digitalcommons.liberty.edu/bio_chem_fac_pubs/105