Optimizing Metagenomics Analysis

Proposal Type

Poster

Presenter Information

Vhuthuhawe MadzingeFollow

Location

Jerry Falwell Library, Lower Esbenshade Atrium

Start Date

11-4-2015 2:00 PM

End Date

11-4-2015 5:00 PM

Comments

Fabich, A. J., Leatham, M. P., Grissom, J. E., Wiley, G., Lai, H., Najar, F., … Conway, T. (2011). Genotype and Phenotypes of an Intestine-Adapted Escherichia coli K-12 Mutant Selected by Animal Passage for Superior Colonization . Infection and Immunity, 79(6), 2430–2439. doi:10.1128/IAI.01199-10

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Apr 11th, 2:00 PM Apr 11th, 5:00 PM

Optimizing Metagenomics Analysis

Jerry Falwell Library, Lower Esbenshade Atrium

It has been known that the tongue is like a rudder that can turn a big ship wherever. It is certainly interesting that something so small can have control over something that is immensely big. Likewise, E.coli is one of the smallest bacteria yet is responsible for large changes in the microbial community of the gastrointestinal tract (GI).

Previous studies have shown that E.coli can improve its colonization in the GI and thus change the populations of other bacteria in the GI; even bacteria bigger than E.coli are greatly impacted by the improvement in colonization of E.coli. The increase in population of E. coli is quite remarkable considering how tiny it is. This experiment is done mice infected with different strains of E.coli, namely MG1655, NIssil, and HS. Thus, microbial community changes are observed in the presence of each of the above mentioned strains .By using E.coli, other species can be identified through the use of the 16s. By becoming knowledgeable about what dwells in the GI tract, may gives us knowledge of how a healthy GI tract looks like.