Category
Poster - Basic
Description
Previous research by Yuan et al. has shown that labeling fluorescent dyes with amino acids is a useful method for fluorescent microscopy because it does not significantly alter the size of the fluorophore. Data by Yuan et al. utilized three different neutral amino acids (glycine, lysine, and proline) and individually linked them to the same fluorophore, resulting in three individual fluorophores. These three fluorophores were tested in the plant, Arabidopsis thaliana, which was able to absorb the fluorophore. Amino acid absorption and transport could be visualized. Fluorophores with the amino acid exhibited enhanced fluorescence when compared to the control fluorophore without an amino acid. In our research, the fluorescent dye 9,10-anthraquinone will be used to which additional side groups, such as amino acids, can be attached. Attaching amino acids to this compound can possibly change its fluorescent properties. Results from attaching an amino acid to anthraquinone will be presented. This amino acid functionalized fluorophore was analyzed by Fourier-transform infrared spectroscopy (FT-IR) and Thin-Layer Chromatography (TLC). Additional analytical techniques include Nuclear Magnetic Resonance (NMR) spectroscopy, electrophoresis, polarimetry, and fluorescence measurements. This research on amino acid labeled fluorescent dyes can lead to further research with selecting other amino acids and fluorescent dyes. Amino acids are inexpensive to purchase and attaching them to a dye could provide a more cost-effective and efficient dye in the biomedical and chemistry field of study.
Analysis of An Amino Acid Labeled Fluorescent Dye
Poster - Basic
Previous research by Yuan et al. has shown that labeling fluorescent dyes with amino acids is a useful method for fluorescent microscopy because it does not significantly alter the size of the fluorophore. Data by Yuan et al. utilized three different neutral amino acids (glycine, lysine, and proline) and individually linked them to the same fluorophore, resulting in three individual fluorophores. These three fluorophores were tested in the plant, Arabidopsis thaliana, which was able to absorb the fluorophore. Amino acid absorption and transport could be visualized. Fluorophores with the amino acid exhibited enhanced fluorescence when compared to the control fluorophore without an amino acid. In our research, the fluorescent dye 9,10-anthraquinone will be used to which additional side groups, such as amino acids, can be attached. Attaching amino acids to this compound can possibly change its fluorescent properties. Results from attaching an amino acid to anthraquinone will be presented. This amino acid functionalized fluorophore was analyzed by Fourier-transform infrared spectroscopy (FT-IR) and Thin-Layer Chromatography (TLC). Additional analytical techniques include Nuclear Magnetic Resonance (NMR) spectroscopy, electrophoresis, polarimetry, and fluorescence measurements. This research on amino acid labeled fluorescent dyes can lead to further research with selecting other amino acids and fluorescent dyes. Amino acids are inexpensive to purchase and attaching them to a dye could provide a more cost-effective and efficient dye in the biomedical and chemistry field of study.
Comments
Undergraduate