Category
Poster - Basic
Description
Danio rerio, commonly known as zebrafish, are well known to have an immune system that mirrors the human immune system. As such, Danio rerio is an excellent biological model for studying the immune system. This research team was attempting to study the activity of B-cells in the immune development of zebrafish. However, the team found that the exact location of B-cells in zebrafish throughout their development is not known. Thus, this research seeks to design a protocol to identify the location of B-cells throughout Danio rerio immune development. The team will be fulfilling this aim by tagging CD79a, also known as Iga, with green fluorescent protein (GFP). Iga is a co-receptor that is continuously expressed on B-cells, and is expressed early in development (Reichlin et al., 2004). Thus, it will be a good target for studying B-cell development. Tagging Iga will be accomplished using a CRISPR Cas-9 delivery system. The CRISPR-Cas9 will be introduced to Danio rerio oocytes via microinjection. The oocytes will be fertilized, and the embryos will be raised to adulthood. Throughout their development, some of the embryos will be sacrificed and imaged to record the location of the B-cells. This process will begin this semester and will be continued throughout future semesters. The results of this study will help fill the gap in knowledge regarding the location of B-cells in Danio rerio immune development. Results have not yet been obtained; however, the team is currently working on writing protocols and obtaining IACUC approval for this project.
CRISPR Cas9 mediated GFP Tagging of cd79a in Danio rerio
Poster - Basic
Danio rerio, commonly known as zebrafish, are well known to have an immune system that mirrors the human immune system. As such, Danio rerio is an excellent biological model for studying the immune system. This research team was attempting to study the activity of B-cells in the immune development of zebrafish. However, the team found that the exact location of B-cells in zebrafish throughout their development is not known. Thus, this research seeks to design a protocol to identify the location of B-cells throughout Danio rerio immune development. The team will be fulfilling this aim by tagging CD79a, also known as Iga, with green fluorescent protein (GFP). Iga is a co-receptor that is continuously expressed on B-cells, and is expressed early in development (Reichlin et al., 2004). Thus, it will be a good target for studying B-cell development. Tagging Iga will be accomplished using a CRISPR Cas-9 delivery system. The CRISPR-Cas9 will be introduced to Danio rerio oocytes via microinjection. The oocytes will be fertilized, and the embryos will be raised to adulthood. Throughout their development, some of the embryos will be sacrificed and imaged to record the location of the B-cells. This process will begin this semester and will be continued throughout future semesters. The results of this study will help fill the gap in knowledge regarding the location of B-cells in Danio rerio immune development. Results have not yet been obtained; however, the team is currently working on writing protocols and obtaining IACUC approval for this project.
Comments
Undergraduate