School of Health Sciences
Biochemistry and Molecular Biology
bacteria, microbiology, transformation, metabolism, diabetes, lactose intolerance, intestine, mammal
Bacteriology | Biotechnology | Cellular and Molecular Physiology | Genetics | Human and Clinical Nutrition | Molecular Biology | Structural Biology
Kader, Bryar P., "The Effect of Transformed Escherichia coli on the Mouse Intestine Microbiome: the Microbial Metabolic Enhancement Hypothesis" (2016). Senior Honors Theses. 613.
Metabolic disorders affect around thirty-four percent of the population in the United States. Among these disorders is lactose intolerance, which results from diminished production of the human lactase enzyme. This disorder and others like it are genetically determined and cannot be cured. However, the use of transformed bacteria implanted in the colon may provide a means by which the faulty pathway can be bypassed. To test whether transformed bacteria have the capability to aid in the digestion of normally indigestible compounds, a transformed strain of Escherichia coli overexpressing the beta-galactosidase enzyme encoded by the lacZ gene was colonized in the mouse intestine to enhance lactose digestion. The experiment provides the platform for similar research to be conducted in the future.