Senior Honors Theses

Publication Date

2015

School

School of Health Sciences

Major

Biology: Cell and Molecular Biology

Keywords

Alzheimer's disease, gene expression, miRNA

Disciplines

Cell Biology | Genetic Processes | Genetics | Medical Cell Biology | Medical Genetics | Medical Molecular Biology | Medical Neurobiology | Molecular and Cellular Neuroscience | Molecular Genetics

Abstract

Previous studies have established a causative role for altered gene expression in development of Alzheimer’s disease (AD). These changes can be affected by methylation and miRNA regulation. In this study, expression of miRNA known to change methylation status in AD was assessed by qPCR. Genome-wide expression changes were determined by RNA-sequencing of mRNA from hippocampus and blood of control and AD mice. The qPCR data showed significantly increased expression of Mir 17 in AD, and sequencing data revealed 230 genes in hippocampus, 58 genes in blood, and 8 overlapping genes showing significant differential expression (p value ≤ 0.05). Expression data from this study revealed novel gene expression changes affecting AD and identified changing genes in blood as potential AD biomarkers.