Publication Date
Summer 6-7-2024
School
School of Health Sciences
Major
Biochemistry and Molecular Biology
Keywords
peroxidase, peroxygenase, enzyme assay, horseradish, pumpkin, butternut squash, jalapeno, guaiacol assay, HPLC, plant peroxidases, dye decolorization, water polution
Disciplines
Agricultural Science | Biochemistry | Environmental Health | Other Plant Sciences | Terrestrial and Aquatic Ecology
Recommended Citation
Hess, Sally, "A Library of 39 Plant Peroxidases: Plants from The Families Cucurbitaceae and Brassicaceae Show Promising Peroxidase and Peroxygenase Activity" (2024). Senior Honors Theses. 1440.
https://digitalcommons.liberty.edu/honors/1440
Abstract
Peroxidases are enzymes that reduce reactive oxygen species (ROS) and oxidize organic substances in living cells. The textile industry, wastewater treatment plants, immunological laboratories, and diagnostic laboratories have extensively used horseradish peroxidase for their oxidating needs. This research aims to provide a tool for identifying potentially useful sources of peroxidases in addition to specifically pinpointing a novel peroxygenase in jalapeño seeds. By using two widely accepted peroxidase assays, the guaiacol assay and the fluorophenol assay, fruits of the genus Cucurbita and roots or leaves of the family Brassicaceae were seen to have high peroxidase activity. Additionally, novel peroxygenase activity was discovered in jalapeño seeds. As a result, an informative library of peroxidases was created.
Included in
Agricultural Science Commons, Biochemistry Commons, Environmental Health Commons, Other Plant Sciences Commons, Terrestrial and Aquatic Ecology Commons
